Nuclear Factor-kappa B inhibition in mouse aortic smooth muscle cells stimulated by cholesterol overload or tumor necrosis factor

ABSTRACT: To test whether cholesterol overload in smooth muscle cells (SMCs) drives NF-kappaB signaling and to map target genes controlled by NF-kappaB signaling in SMCs, we cultured primary aortic SMCs from wildtype mice in the presence of cyclodextrin-complexed cholesterol or the prototypical NF-kappaB activator tumor necrosis factor (TNF) with or without blocking canonical NF-kappaB signaling by knockdown of inhibitor of kappaB kinase beta (Ikbkb).

INSTRUMENT(S): DNBSEQ-G400

ORGANISM(S): Mus musculus

SUBMITTER:  

PROVIDER: E-MTAB-13416 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-12069

REPOSITORIES: biostudies-arrayexpress