5'-RACE-Seq analysis of Salmonella hilD and leuO promoters' response to adjacent transcription

ABSTRACT: Gene silencing by nucleoid-associated protein H-NS is sensitive to neighbouring transcription. This effect does not require neighbouring transcription to elongate into the silenced region. Here we measured how activating transcription of a tetRA cassette positioned upstream of either hilD or leuO promoters of Salmonella affected the activity of these promoters. The tetRA cassette included a strong Rho-independent transcription terminator at the 3' end of tetA. Transcription of the cassette was activated with anhydrotetracycline (AHTc). The activities of hilD and leuO promoters were measured upon performing template-switching reverse transcription follwed by semi-quantitative PCR and Illumina high-throughput sequencing.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Salmonella enterica subsp. enterica

SUBMITTER: Lionello Bossi 

PROVIDER: E-MTAB-13482 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress