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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq files of Virulent and Avirulent EPEC microcolonies from aerobic, butyrate, infant stool metabolites and anaerobic conditions

ABSTRACT: EPEC wild type E2348/69 (strain O127:H6) bacteria were grown overnight (ON) in LB medium (Sigma-Aldrich) at 37°C without shaking, diluted 1:40 into DMEM-HEPES (Gibco, Israel) medium and incubated for 3h at 37°C without shaking to exponential phase (optical density (OD) ~ 0.6). Bacteria were plated on LB plate with streptomycin (50 µg/ml), incubated at 32°C for 15h (for aerobic LB) or for 12h (for aerobic butyrate and infant stool metabolites conditions) or at 37°C (for anaerobic condition) for 12 h and colonies of two sizes (Virulent & Avirulent) were sampled, RNA was extracted and sequencing libraries were constructed based on the RNAtag-Seq protocol (Shishkin et al., 2015) with several modifications.

INSTRUMENT(S): NextSeq 550, RNAtag-Seq protocol (Shishkin et al., 2015)

ORGANISM(S): Escherichia coli

SUBMITTER: Raya Faigenbaum-Romm

PROVIDER: E-MTAB-13486 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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