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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ATAC-seq of WT mouse beige ME3 cells on days 0, 1, and 7 of adipogenic differentiation

ABSTRACT: A map of open chromatin in WT beige ME3 preadipocytes cells on days 0, 1 and 7 of adipogenic differentiation. 100.000 cells were collected in duplicates at each timepoint by trypsination. Cells were slowly frozen at -80C in culture media containing 5% DMSO before shipping on dry ice to Active Motif for open chromatin profiling. In brief, chromatin was isolated from the cells followed by Tn5 transposase-mediated insertion of sequencing primers into open chromatin regions. After library preparation, high-throughput sequencing (Illumina) was performed and reads were then aligned to the mm10 genome using Bowtie2 (Version 2.3.4.3)..

INSTRUMENT(S): NA, Active Motif Catalog No. 53150, Illumina HiSeq 3000

ORGANISM(S): Mus musculus

SUBMITTER: Jan-Inge Bjune

PROVIDER: E-MTAB-13520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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