Heart RNA-Seq of cardiomyocyte-specific and inducible Prmt5 knockout mouse model

ABSTRACT: Our study introduces a novel animal model featuring a cardiomyocyte-specific, inducible knockout of the Prmt5. The primary aim of this model is to investigate the regulatory mechanisms of PRMT5, the main representative of type II PRMTs. We found that PRMT5 controls Acadvl mRNA splicing and that disruptions in this regulation lead to loss of functional ACADVL and cardiac dysfunction. Importantly, we also show that maintained expression of ACADVL is cardioprotective highlighting a potential therapeutic strategy. The animal model was created using a conditional-ready allele of Prmt5 sourced from the European Mouse Mutant Archive (EMMA, specifically the B6Brd;B6N-Tyrc-Brd Atm1Brd Prmt5tm2a(EUCOMM)WtsiPrmt5 strain). To achieve a conditional knockout, the lacZ reporter was excised using flippa

INSTRUMENT(S): BGISEQ-500, NextSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Thiago Britto Borges 

PROVIDER: E-MTAB-13594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress