Project description:AdpA is transcription regulator and controls gene expression. The goal of RNA-seq experiment was to determine AdpA regulonsduring growth in liquid medium. Dataset contains two strains (adpA deletion mutants and wild type), two time points (12 and 20h).
Project description:The BldC gene is required for the formation of aerial hyphae in Streptomyces venezuelae. It is a 68 amino acid DNA-binding protein related to the MerR family of transcription factors. BldC deletion strains are bald because they initiate sporulation prematurely, omitting the formation of aerial hyphae altogether. This RNA-Seq experiment was carried out to determine and quantify effect of BldC on the expression of all genes in the S. venezuelae genome after 10 and 14 hours of culture.
Project description:Mitomycin C is a DNA crosslinking agent. This experiment was carried out to determine the effect of Mitomycin C on genome wide transcription in Streptomyces venezuelae NRRL B65442.
Project description:BldB is a regulatory protein in Streptomyces and most species have a number of paralogs in their genomes. Deletion of BldB leads to a developmental deficiency and the colonies fail to produce aerial mycelium. In this experiment global gene expression was compared between the WT and BldB deletion strain of Streptomyces venezuelae NRRL B65442 after 10, 14 and 22 hours of growth in liquid cultures.
Project description:Expression of iosA is greatly activated in the bldB deletion strain. whiJ9 is divergently transcribed from iosA and the double mutant (whiJ9 + bldB) was made to determine if WhiJ mediates the activation of iosA in the absence of BldB.
Project description:We studied the binding of ParB protein to Streptomyces venezuelae chromosome during development using native ParB and anti-ParB antibody. Additionally investigated the role of SMC protein in ParB DNA binding using smc deletion strain.
Project description:Transcription profiling by array of Streptomyces venezuelae wild type and bldN mutant strains during a time course from 8 to 20 hours, sampling at 2 hour intervals.
Project description:We studied the binding of HupS protein to Streptomyces venezuelae chromosome during development of aerial hyphae (14 hour of growth) using HupS-FLAG protein. Additionally investigated the role of SMC protein in HupS DNA binding using smc deletion strain.
Project description:We studied the binding of SMC protein to Streptomyces venezuelae chromosome during development of aerial hyphae (14 hour of growth) using SMC-FLAG protein. Additionally investigated the role of ParB protein in SMC DNA binding using parB deletion strain.