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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ScRNA-seq of mouse pancreatic islets from non-transgenic and pericyte MyD88-mutated mice

ABSTRACT: To elucidate the role of pericytic cytokines in islet inflammation, we interfered with the expression of MyD88, a key modulator of the IL1R1/TLR pathway, in pericytes using transgenic mice (Nkx3.2-Cre;MyD88 flox/flox) . To determine the impact on islet immune cells, we isolated CD45+ cells from the islets of both non-transgenic and Myd88-deficient mice via MACS, pooling samples from 4-5 littermate male mice. Additionally, unsorted islet cells from the same pool were included to meet the cell number requirements for sample preparation using X10 genomics.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Limor Landsman

PROVIDER: E-MTAB-13640 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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