Project description:Mus musculus macrophages were derived from bone marrow, and treated with the supernatant of of DYRK1B-deletion (multiple clones) or Wildtype mKpc4 cells.
Project description:Effect of LPA-Mixture (16:0, 18:0 18:1 18:2, 20:4) and LPAR2 specific (H2L5186303) or LPAR1 specific (Ro6842262) Inh. in OC91s cells.
Project description:Natural killer cells were exptracted from PMBCs of healthy donors, exposed to arachidonic acid, Il-2, both, or ascites and their expression changes identified via RNAseq (QuantSeq).
Project description:A Proliferation Inducing Ligand (APRIL) is a member of the tumour necrosis factor (TNF) superfamily and has recently been shown to modulate pro-inflammatory astrocyte responses, as well as to ameliorate disease outcome in the experimental autoimmune encephalomyelitis mouse model for multiple sclerosis (MS). We demonstrated that adeno-associated virus (AAV)-mediated delivery of APRIL was able to rescue immune-stimulated murine iPSC-derived neurospheroids from detrimental cell death and neurodegenerative processes. In addition, T2 and diffusion weighted MRI with histological confirmation demonstrated that AAV-mediated secretion of APRIL by cortical neurons could reduce cuprizone-induced neuro-inflammation and/or demyelination in the splenium of the corpus callosum. To further document the beneficial effect of APRIL, a transcriptome-proteome integration study for the splenium was performed that revealed the activation of cellular pathways associated with alternative immune cell polarisation, cell survival and neuroregeneration. Here, the associated transcriptome data (FASTQ files and BAM files) are deposited.
Project description:RNA-seq of differentiated organoids (day 7) from a patient with an AGR2 mutation, patients with ulcerative colitis, or non-IBD controls. After 6 days differentiation, organoids were treated for 24 hours with Tunicamycin (T7765, Sigma Aldrich,1 µg/ml) or vehicle control (0.1 % DMSO).
Project description:Patient-derived intestinal organoids provide an excellent tool to unravel mechanisms underlying ulcerative colitis (UC). Fresh biopsies, to isolate crypts and culture organoids, were obtained from both inflamed and non-inflamed regions from eight patients with active UC (Mayo endoscopic subscore ≥2), and from eight non-IBD controls.To address the inflammatory character of ex vivo organoids, we compared the transcriptome of biopsies, crypts and organoids derived from inflamed, and non-inflamed regions and aimed to (re-)induce inflammation ex vivo.