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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single cell RNA-Seq expression profiling of RFP-positive cells isolated from Ki67-RFP/Tcf7l2/Villin-CreERT2 mouse small intestinal epithelium 7 days after tamoxifen administration

ABSTRACT: The T-cell factor 4 (Tcf4) protein (encoded by the Tcf7l2 gene) is a nuclear transcription regulator, which plays a key role in maintaining intestinal homeostasis, stem cell self-renewal and epithelial cell differentiation. Tcf4 has been identified as a key regulator of Paneth cell function in small intestinal crypts. It controls the expression of antimicrobial peptides and other effector molecules that contribute to the maintenance of the intestinal microbiota and protection against intestinal pathogens. Depletion of Tcf4 in mouse intestinal epithelial cells leads to loss of proliferating cells and dramatic changes in the morophology of the intestinal epithelum. To examine Tcf4 function in more detail, we used the Ki67-RFP Tcf7l2-flox/flox Villin-CreERT2 mouse strain. In this strain, proliferating cells are marked by expression of red fluorescent protein (RFP) and Tcf4 depletion from intestinal epithelial cells is accomplished by administration of tamoxifen. After 7 days, small red structures, i.e. \\"escaped crypts\\", appear in the epithelium, marking proliferative zones that appear to have escaped Tcf4 deletion. We performed gene expression profiling of RFP+ cells from these \\"escaped crypts\\" and from crypts isolated from wild-type epithelium.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Jan Kubovčiak

PROVIDER: E-MTAB-13731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profiling of RFP-positive cells isolated from Ki67-RFP/Tcf7l2/Villin-CreERT2 mouse small intestinal epithelium 7 days after tamoxifen administration.

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