Mycobacterium tuberculosis efpA CRISPRi knock-down transcriptional profile - induced efpA knock-down strain vs non-induced condition

ABSTRACT: In Mycobacterium tuberculosis, the efflux pump, EfpA, plays an essential but uncharacterised role in its physiology and antibiotic tolerance. In this study, an ATc-inducible CRISPR interference (CRISPRi) system was introduced into M. tuberculosis to knock-down the expression of efpA and determine any subsequent effects on the organism. As part of the study, microarray experiments were performed to determine any changes in gene expression caused by efpA repression M. tuberculosis by comparing the transcriptomic profile between ATc-induced efpA knock-down cultures to that of efpA knock-down cultures without ATc-induction.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Adam Roberts 

PROVIDER: E-MTAB-13755 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress