Regulation of glycolytic activity in multiple cell types explains the ability of FDG-PET to measure disease activity in atherosclerosis

ABSTRACT: The aim of the single cell RNASeq experiment is to explore the molecular changes in the progressing and regressing plaques of an atherosclerosis pig mode and specifically the long-term changes glycolysis pathway. To produce these glycolysis signatures, we randomized male and female pigs into two experimental groups: an actively progressing atherosclerosis group subjected to 15 months of HFHC diet (HFHC group f, n=4; m, n=2), and an LF/MTPi group fed 12 months of HFHC diet and then transitioned to low-fat diet with MTP inhibitor for three months (f, n=4; m, n=1). At the time of euthanasia, plaque from the distal 0.5 cm of the abdominal aorta was quickly processed and analyzed by scRNA-sequencing.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Sus scrofa

SUBMITTER: Carlos Torroja 

PROVIDER: E-MTAB-13898 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress