Engineering Material Properties of Transcription Factor Condensates to Control Gene Expression in HEK-293T Cells

ABSTRACT: Phase separation of biomolecules into condensates is a key mechanism in the spatiotemporal organization of biochemical processes in cells. We systematically engineered light-inducible transcription factor condensates with different material properties and analyzed their influence on transcription activation. When transcription factor condensates were transformed into solid-like gels, we observed a reduced activation of gene expression. We wanted to evaluate the impact that the condensate formation of the transcription factor RelA had on its endogenous promoters using expression data. To this aim, HEK-293T cells were transfected either with empty vector (1-3) or eGFP-RelA (4-6) or eGFP-RelA, Cry2olig-mCh-FUSN-NLS-NbGFP and Cry2olig-mCh-FUSN-NLS, along with an NF-κB-responsive SEAP reporter

INSTRUMENT(S): DNBSEQ-G400, DNBSEQ Devices, TriFast (VWR, 30-2010), RNeasy Mini Kit (Qiagen, 74104), DNAse 1 (NEB, M0303); Agilent 2100 Bioanalyzer, HEK-293T cells, Media, PEI, Illumination device

ORGANISM(S): Homo sapiens

SUBMITTER: Stefan Lohse 

PROVIDER: E-MTAB-13905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress