Genome-wide DNA methylation profiling of regenerating ear pinnae in BALB/c mice induced with pharmacological epigenetic therapy involving subcutaneous administration of zebularine and retinoic acid in alginate hydrogel

ABSTRACT: Aim The experiment aimed to analyse genome-wide DNA methylation re-patterning in regenerating ear pinnae in mice in response to zebularine and retinoic acid treatment. Animals The experiments were conducted on 8 to 10-week-old females of the BALB/c mouse strain in the Tri-City Academic Laboratory Animal Centre. After the mice were anaesthetised with isoflurane, through-and-through holes of 2 mm diameter were made in the ear pinna centre using a scissor-style ear punch (Hammacher Solingen; LOT FTC-15/8670/1). Next, the animals were randomised into groups of six. Immediately after wounding, the mice were administered subcutaneous injections of alginated hydrogel formulations. Mice in one group received zebularine (48 mg in 200 µl of 2% alginate hydrogel) and retinoic acid (0.8 mg in 200 µl

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Paweł Sachadyn 

PROVIDER: E-MTAB-13955 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress