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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA sequencing of control and OsPRK1/2/3 triple knockout mutant in mature rice anthers (Oryza sativa japonica)

ABSTRACT: We created a triple loss-of-function/knockout mutant targeting three rice (Oryza sativa japonica) genes simultaneously via the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/Cas9 system. The three selected genes are as follows: OsPRK1 (LOC_Os08g40990), OsPRK2 (LOC_Os06g45240), and OsPRK3 ((LOC_Os02g07810). These three OsPRKs are strongly transcriptional expressed in the mature anthers of rice (stages 13) and bi-/tricelluler pollen. The triple mutant of these OsPRKs does not produce self-fertilized seeds due to defects in the hydration and germination of pollen grains (male-sterile). This data is about RNA-sequencing transcriptome data about the Oryza sativa Dongjin used as a control and triple mutant of OsPRKs (OsPRK1/2/3). We sampled mature anther for the analysis.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Eui-Jung Kim

PROVIDER: E-MTAB-13984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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