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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Proof of principle for iPS2-10X-seq: cardioids experiment during 3D cardiac differentiation

ABSTRACT: Proof of principle and optimization experiment on cardioid with10X-based-iPS2-seq was performed on two batches of hPSC-derived cardioids at day 7.5 and pooled together after sample multiplexing. hPSC were primed and treated with Tetracycline 5 days prior cardiac differentiation. CG000388 Rev B (when multiplexing) User Guide was followed with sample multiplexing, provided information of the multiplexing for the cellranger aggr is provided. Sequencing has been performed on a Nextseq 1000 on a P2 Illumina kit 100 cycles. Sequencing run: Read1, 28 cycles; Index1, 10 cycles; Index2, 10 cycles, Read2, 90 cycles.

INSTRUMENT(S): NextSeq 1000

ORGANISM(S): Homo sapiens

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PROVIDER: E-MTAB-14066 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-14065

REPOSITORIES: biostudies-arrayexpress

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