Bulk RNA-seq profiling the response of human first-trimester and term Hofbauer cells to Listeria monocytogenes infection in vitro

ABSTRACT: To understand the response of human Hofbauer cells to Listeria monocytogenes infection and how it differs between first trimester and term, we established an infection assay using primary human first-trimester and term Hofbauer cells isolated by fluorescence-activated cell sorting and cultured in vitro for 7 days. We then profiled and compared the transcriptomes of control cells (treated with PBS or Heat-Killed Listeria monocytogenes) and cells infected with Listeria monocytogenes for 8 hours using the NEBNext® Ultra II Directional RNA Library Prep Kit (Illumina).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Qian Li 

PROVIDER: E-MTAB-14086 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress