Project description:Cognitively normal brains are compared to sporadic AD and Down syndrome brains with AD for comparison of two different forms of Alzheimer's disease
Project description:C57BL/6 Mouse cortex samples at 2-months of age, both sexes, exposed to one acute 5-hour dose of 100ug/m3 of air pollution from diesel exhaust particles or dust from the World Trade Center collapse. C57BL/6 female mouse cortex exposed to one acute 4-hour dose of 500ug/m3 of woodsmoke.
Project description:U87 cells overexpressing ApoE isoforms were treated with sulforaphane for 18 hours for comparison with a DMSO control. ApoEChimp is on the ApoE4 backbone but contains R61T which is thought to be the isoform defining residue.
Project description:The actin cytoskeleton is a highly conserved structural network that supports diverse cellular processes, but its contribution to organismal aging is not well understood. This project investigates how genetic and pharmacological perturbations of actin and actin-binding proteins influence global gene expression and aging phenotypes in Caenorhabditis elegans. Whole-animal RNA interference was used to knock down key actin regulatory genes, including arx-2 (Arp2/3 complex), unc-60 (cofilin), and lev-11 (tropomyosin). Here Bulk RNA sequencing was performed to assess transcriptional responses to actin cytoskeletal disruption using genetic knockdown (RNAi) of actin-binding proteins and actin de/stabilizing molecules. These datasets capture transcriptomic signatures associated with actin dysfunction, aging trajectories, and stress responses, and provide a resource for exploring the molecular links between cytoskeletal integrity and longevity regulation.
Project description:SAT1 is the mammalian polyamine acetylation enzyme. In order to characterize the transcriptional alterations resulting from SAT1 ablation in tumor cells in vivo, we knocked out SAT1 in a genetic glioma model (PTEN/P53/NF1 knockout) and performed RNAseq.
Project description:The transcriptomic innate immune response derived from human nasal epithelial cells depends on how Streptococcus pneumoniae colonises the nasopharynx. This study compared three wild type strains and one deficient in pneumolysin to explore the pathways of epithelial activation following a three hour infection in vitro.
Project description:Microglia are increasingly recognized as active drivers of Parkinson's disease (PD), contributing to neuroinflammation, α-synuclein clearance, and dopaminergic neuron loss. Because aging is the strongest risk factor for PD and independently shifts microglia toward a primed, pro-inflammatory state, disentangling age- and disease-related transcriptional programs is essential. Aim and workflow This dataset profiles the bulk transcriptome of microglia from [PD model] and control mice at [young] and [old] ages (2×2 design) to resolve disease, age, and interaction effects. Brains were enzymatically dissociated, microglia isolated by MACS using CD11b MicroBeads, and total RNA extracted for bulk RNA-seq library preparation and sequencing on Novaseq 6000. n = 3-4 per group. Both sexes included. All samples except Sample1 includes two mice. Sequencing was done with a target of 40G per sample PE150.
Project description:We wanted to explore the function of MG53 in THP-1 cells, so we treated THP-1 cells with control b-gal or MG53 adenovirus, and using PMA to induce THP-1 differentiated into macrophages. Thus exploring how MG53 affect macrophages.
Project description:We wanted to explore the function of MG53 in THP-1 cells, so we treated THP-1 cells with control b-gal or IRF7 adenovirus, and using PMA to induce THP-1 differentiated into macrophages. Thus exploring how MG53 affect macrophages.