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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc

ABSTRACT: RpfB is one of five resuscitation promoting factors in Mycobacterium tuberculosis. We previously published data indicating the 5' UTR of the gene contains a regulatory riboswitch with an unknown (at the time) ligand. We overexpressed the switch in Mycobacterium smegmatis (which does not contain the rpfB gene), reasoning that overexpression of the switch would s sequester the unknown ligand, giving rise to a phenotype that would help us identify it.

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Mycobacterium smegmatis str. MC2 155

SUBMITTER: Peter Polgar

PROVIDER: E-MTAB-14260 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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