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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The impact of microbial colonisation on intestinal macrophages

ABSTRACT: In this study we compared three different groups of mice to understand the role of antibiotics and microbial colonisation during early life. (1) young mice that were untreated and sacrificed pre-weaning, 12 days after birth, (2) control mice were also untreated and sacrificed 44 days after birth, these mice were were weaned at 24 days old and (3) recolonised mice that were treated with broad-spectrum antibiotics (ampicillin, vancomycin, neomycin and metronidazole) from 14 days to 35 days of age. At 37 days mice were gavaged with faecal microbial matter prepared from control mice before being sacrificed at 44 days of age. They were also weaned at 24 days old.

INSTRUMENT(S): 10x Genomics Chromium, Illumina NovaSeq 6000, manual

ORGANISM(S): Mus musculus

SUBMITTER: Aleksandra Kolodziejczyk

PROVIDER: E-MTAB-14263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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