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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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HAND1 ChIP-seq at day 3 of cardiac differentiation from human embryonic stem cells

ABSTRACT: The aim was to identify HAND1 binding sites at low and high HAND1 levels at an early stage of hESC cardiac differentiation. We generated a HAND1-AM-Tag (Active Motif) knock-in to enable sensitive and specific ChIP-seq analysis. The hESCs were differentiated in 3D embryoid bodies with BMP4, Activin A and CHIR. To increase HAND1 levels, we treated one population with SB431542 from day 2. Samples were harvested on day 3 for ChIP.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER:

PROVIDER: E-MTAB-14280 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP162226

REPOSITORIES: biostudies-arrayexpress

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