RNA-seq of thermoacidophilic crenarchaeon Sulfolobus acidocaldarius grwon with different glycerol concentrations reveals an unusual glycerol-3-phosphate dehydrogenase

ABSTRACT: To elucidate the glycerol catabolism in glycerol-adapted S. acidocaldarius MW00G the carbon source dependent global transcriptional response to glycerol compared to D-xylose the transcriptome was analyzed via RNA-Seq. Growth experiments with the S. acidocaldarius MW00G werewas performed in triplicates at 76°C and 120 rpm under constant shaking using (Innova®44, (New Brunswick, Germany) in the presence 10 mM glycerol, 20 mM glycerol or 40 mM glycerol as sole carbon and energy source. For comparison the same strain was cultivated with 0.2 % (w/v) D-xylose. RNA isolation, library preparation, and next-generation cDNA sequencing RNA was isolated using Zymo Direct-zol RNA Miniprep kit following manufactures instructions. The RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and t

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Sulfolobus acidocaldarius

SUBMITTER: Tobias Busche 

PROVIDER: E-MTAB-14293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress