RNA-Seq of An Integrated Signaling Network Controls P-Bodies Dynamics and mRNA Translation in Human Glioblastoma

ABSTRACT: Processing (P)-bodies are cytoplasmic membrane-less condensates including translationally repressed mRNAs and proteins involved in the mRNA decay machinery. The mechanisms underlying P-bodies nucleation and mRNA decay have been largely described. However, the impact of signaling networks in P-bodies dynamics and mRNA translation is still not well understood. Here, we identify a ribonucleoprotein network assembled by the E3 ubiquitin ligase Praja2 at P-bodies that coordinates protein translation and mRNA turnover in human glioblastoma (GBM). Praja2 physically interacts and spaBally co-localizes with the RNA helicase DDX6, a core component and nucleating factor of P-bodies. Stimulation of the G protein-coupled receptor (GPCR)-cAMP pathway in GBM cells induces a non-proteolytic polyubiquityla

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Homo sapiens

SUBMITTER:  

PROVIDER: E-MTAB-14326 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP162932

REPOSITORIES: biostudies-arrayexpress