CRISPR-MIP replaces PCR and reveals GC and oversampling bias in pooled CRISPR screens

ABSTRACT: This is data for the evaluation of a new way of counting sgRNAs in CRISPR screens using padlock probes and UMIs. It is compared to the typical PCR-based approach. In particular, a dropout screen was performed in MiaPaCa-2 cells using the Human Kinome CRISPR pooled library (Addgene #75314)

INSTRUMENT(S): NextSeq 550

ORGANISM(S): synthetic construct

SUBMITTER: Johan Henriksson 

PROVIDER: E-MTAB-14379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress