Human skeletal muscle fibroblasts and stem cells cultured with rat motor neuron cells
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ABSTRACT: The study was designed to compare whether and how muscle fibroblasts (FIB) and muscle stem cells (MYO) interact with motor neurons. Moreover, we aimed to investigate whether muscle cells, isolated from lifelong exercisers, exerted a protective and supportive effect on motor neurons. These objectives were investigated in vitro using primary muscle cells from human donors and primary motor neurons from rat embryos and were analyzed by immunocytochemistry and species-specific RNA sequencing. A short period (24 hours) of co-culture was chosen for the RNA seq experiment, as differences on the gene expression level were expected to be greater initially. This accession contains the counts from the human RNA sequences.
INSTRUMENT(S): Qubit 4.0 Fluorometer/ Agilen 5300 Fragment Analyzer, Illumina NovaSeq 6000
ORGANISM(S): Homo sapiens
SUBMITTER: Peter Schjerling
PROVIDER: E-MTAB-14395 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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