Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single cell RNA sequencing data of the in vitro lifecycle of the kinetoplastid parasite Trypanosoma cruzi

ABSTRACT: Single cell RNA sequencing data across seven samples of the Trypanosoma cruzi in vitro lifecycle. The samples have the following lifecycle stage compositions: Sample 1 (fastq file prefix = cycle_epimast) - Mix of exponential growth growth epimastigotes and amastigotes derived 120 hours post infection of Vero cells with trypomastigotes. Sample 2 (fastq file prefix = cycle_amast) - Mix of amastigotes derived 6 and 24 hours post infection of Vero cells with trypomastigotes. Sample 3 (fastq file prefix = cycle_metacyc1) - Mix of stationary growth phase epimastigotes and metacyclic trypomastigotes (first of two biological replicates). Sample 4 (fastq file prefix = cycle_metacyc2) - Mix of stationary growth phase epimastigotes and metacyclic trypomastigotes (second of two biological replicates). Sample 5 (fastq file prefix = cycle_trypomast)- Trypomastigotes harvested from culture 7 days post infection of Vero cells with trypomastigotes. Sample 6 (fastq file prefix = LifeCycleMix1) - A mix of amastigotes derived 6, 24 and 120 hours hours post infection of vera cells with trypomastigotes, exponential and stationary growth phase epimastigotes, metacyclic trypomastigotes and trypomastigotes harvested from culture 7 days post infection of Vero cells with trypomastigotes (first of two biological replicates). Sample 7 (fastq file prefix = LifeCycleMix2) - A mix of amastigotes derived 6, 24 and 120 hours hours post infection of Vero cells with trypomastigotes, exponential and stationary growth phase epimastigotes, metacyclic trypomastigotes and trypomastigotes harvested from culture 7 days post infection of Vero cells with trypomastigotes (first of two biological replicates). Sample 8 (fastq file prefix = A2, B2, C2 and D2) - Amastigotes derived 48 hours post infection of Vero cells

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Trypanosoma cruzi

SUBMITTER: Ross Laidlaw

PROVIDER: E-MTAB-14406 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Oral transmission of T. cruzi is probably the most frequent mechanism among animals in the wild. In this context, there is a high prevalence of human infections in regions where triatomine infection with T. cruzi is low. This led to the hypothesis that the consumption of raw or undercooked meat from animals infected with T. cruzi could be responsible for the transmission of the infection. Therefore, the general objective of this study was to demonstrate the role of meat consumption from infected animals in the oral transmission of T. cruzi infection. Groups of five female mice Balb/c were fed with muscles obtained from mice in the acute phase of infection by the clone H510 C8C3hvir of T. cruzi, and the infection of the fed mice was monitored by a parasitemia curve. Similarly, we assessed the infective capacity of T. cruzi trypomastigotes and amastigotes by infecting groups of five mice Balb/c females, which were infected orally using a nasogastric probe, and the infection was monitored by parasitemia curve. Finally, different trypomastigote and amastigote inoculums were used to determine their infective capacity. Adhesion assays of T. cruzi proteins to AGS stomach cells were performed and the adhered proteins were detected by western blotting using monoclonal or polyclonal antibodies. In all cases, 60–100% of animals were fed meat from mice infected in the acute phase or infected by means of a nasogastric probe with trypomastigotes or amastigotes. These animals developed high parasitemia, and 80% died around day 40 post-infection. The adhesion tests showed that cruzipain is a molecule of trypomastigotes and amastigotes that binds to AGS cells. LC-MS/MS also confirmed that transialidase may be involved in TCT attachment of TCT or invasion of stomach cells. It was concluded that the consumption of meat from infected animals in the acute phase of T. cruzi infection allows transmission of the infection. Similarly, trypomastigotes and amastigotes were able to infect mice when administered orally, while cruzipain was a relevant molecule in this infective process.

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Single cell RNA sequencing data of the in vitro lifecycle of the kinetoplastid parasite Trypanosoma cruzi

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