Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq analysis of RpoS in Vibrio cholerae

ABSTRACT: The experiment contains ChIP-seq data for an rpoS- version of Vibrio cholerae strain A1552, or a derivative encoding rpoS-3xFLAG. In both cases, smooth colony variants were used. The strains were both grown at 37 degrees, in LB medium, to an OD600 of 2.0, and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Vibrio cholerae

SUBMITTER: David Grainger

PROVIDER: E-MTAB-14434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

ChIP-seq analysis of the Vibrio cholerae VpsT protein

Project description:The experiment contains ChIP-seq data for Vibrio cholerae strain E7946. The strain was grown at 37 degrees in LB medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

2021-11-18 | E-MTAB-10829 | biostudies-arrayexpress

ChIP-seq analysis of the Vibrio cholerae AphA protein

2019-10-21 | E-MTAB-7953 | biostudies-arrayexpress

ChIP-seq analysis of genome-wide DNA binding by transcription factors HapR and LuxO in Vibrio cholerae

2023-03-29 | E-MTAB-11906 | biostudies-arrayexpress

ChIP-seq of the cyclic-AMP Receptor Protein (CRP) and RNA polymerase sigma 70 subunit in Vibrio cholerae

Project description:The experiment contains ChIP-seq data for Vibrio cholerae strain N16961. The strain was grown at 37 degrees in M9 minimal medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using no antibodies (mock immunoprecipitations) or antibodies against CRP or the RNA polymerase sigma 70 subunit. Libraries were prepared using DNA remaining after immunoprecipitation.

2019-02-04 | E-MTAB-6472 | biostudies-arrayexpress

Chip-seq analysis of NusA and Rho binding in Escherichia coli with or without H-NS

Project description:ChIP-seq data for Escherichia coli strain MG1655 and MG1655 hns::kan. The strains were grown in LB medium at 37 degrees to log phase and crosslinked with 1% (v/v) formaldehyde. After sonication, to lyse cells and fragment DNA, immunoprecipitations were done using anti-NusA and anti-Rho antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

2026-05-20 | E-MTAB-16394 | biostudies-arrayexpress

ChIP-seq analysis of MarA, SoxS, Rob and RamA biding across the Salmonella Typhimurium SL1344 genome

Project description:ChIP-seq data for Salmonella Typhimurium strain SL1344. The strain was grown at 37 degrees in LB medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies (for SoxS, Rob and RamA) or anti Myc (for MarA) antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

2023-03-29 | E-MTAB-12627 | biostudies-arrayexpress

ChIP-seq analysis of Escherichia coli sigma 38 binding in cells lacking H-NS

Project description:The experiment contains ChIP-seq data for Escherichia coli strain RPB104 hns::kan. The strain was grown at 37 degrees in LB medium to stationary phase and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies. Libraries were prepared using DNA remaining after immunoprecipitation.

2021-03-01 | E-MTAB-8778 | biostudies-arrayexpress

ChIP-seq of the multiple antibiotic resistance repressor (MarR) protein of Enterotoxigenic Escherichia coli strain H10407

Project description:The experiment contains ChIP-seq data for Enterotoxigenic Escherichia coli strain H10407 transformed with plasmids pAMNF (encoding an N-terminal MarR-3xFLAG fusion) or pAMNM (encoding an N-terminal MarR-8xmyc fusion). The cells was cultured at 37 degrees in LB medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using an anti-FLAG antibody (i.e. the strain encoding the MarR-8xmyc fusion was used as a control). Libraries were prepared using DNA remaining after immunoprecipitation.

2017-12-01 | E-MTAB-5591 | biostudies-arrayexpress

ChIP-seq analysis of H-NS in Acinetobacter baumannii AB5075

Project description:The experiment contains ChIP-seq data for Acinetobacter baumannii strain AB5075 encoding 3xFLAG tagged H-NS. Experiments were done with or without ectopic expression of the truncated H-NS-39 protein (corresponding to the H-NS multimerization surface). The strain was grown at 37 degrees in LB medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using anti-FLAG antibodies against. Libraries were prepared using DNA remaining after immunoprecipitation.

2024-07-15 | E-MTAB-13790 | biostudies-arrayexpress

RNAseq analysis of Vibrio cholerae A1552 ∆rpoS (∆VC0534) from Smooth and Rugose backgrounds

Project description:RNA-sequencing of Vibrio cholerae A1552 ∆rpoS and parental strains from smooth and rugose backgrounds grown as spots on LB agar

2025-09-07 | GSE255512 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data