ChIP-seq analysis of histone modifications and CCCTC-binding factor (CTCF) binding in the livers of five strains of mouse and rat

ABSTRACT: This study aims to investigate the interactions of mutagenic lesions from diethylnitrosamine (DEN) treatment of mouse and rat livers with cellular processes such as replication, transcription, and the interactions of DNA with proteins. Data are available for the following laboratory strains of mouse and rat: Mus musculus (C57BL/6J and C3H), Mus musculus castaneous (CAST), Mus caroli (CAROLI), and Rattus norvegicus (F344). Liver samples from untreated mice (postnatal day 15, P15) and rats (postnatal day 56, P56) were isolated and flash-frozen. ChIP-seq was performed to identify histone modification (H3K4me3 and H3K27ac) and CTCF binding sites in livers of ten pooled individuals (mice) or one individual (rat). The experiment was done with at least three biological replicates, plus matched input libraries. Data for CTCF binding in C3H mice is available at ArrayExpress (E-MTAB-11959).

INSTRUMENT(S): Illumina MiSeq, Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Masa Roller 

PROVIDER: E-MTAB-14454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress