Gene expression profiles of peritoneal macrophages from Sphingosine 1-phosphate receptor type 1 (S1pr1) overexpressing mice against control mice.

ABSTRACT: Sphingosine 1-phosphate (S1P) is a lysosphingolipid with anti-atherogenic properties, but mechanisms underlying its effects remain unclear. We here analyzed the consequences of overexpressing the S1P receptor type 1 (S1pr1) on peritoneal macrophage gene expression. To amplify S1pr1 signaling in macrophages, double transgenic mice expressing mouse S1pr1 in mononuclear cells were constructed by crossing two lines. The S1pr1-KI line carries a transgenic cassette in the Rosa 26 locus harboring the mouse S1P1 cDNA and separated from the CAG promoter by a lox-Stop-lox insert. S1pr1-KI mice were then crossed to LysMCre mice expressing Cre recombinase under the control of the lysozyme M promoter, which drives macrophage expression. In the offspring the lox-Stop-lox insert is excised in Cre-expres

ORGANISM(S): Mus musculus

SUBMITTER: Ralph Burkhardt 

PROVIDER: E-MTAB-14469 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress