Multi-layer dosage compensation of the avian Z chromosome (single-cell RNA-seq)

ABSTRACT: Sex-chromosome dosage represents a challenge for heterogametic species to maintain correct proportion of gene products across chromosomes in each sex. While therian mammals (XX/XY system) achieve near-perfect balance of X-chromosome mRNAs through X-upregulation and X-inactivation, birds (ZW/ZZ system) have been found to lack efficient compensation at RNA level, challenging the necessity of resolving major gene-dosage discrepancies in avian cells. Through allele-resolved multiome analyses, we comprehensively examined dosage compensation in female (ZW), male (ZZ), and rare intersex (ZZW) chicken. Remarkably, this revealed that females exhibit upregulation of their single Z through increased transcriptional burst frequency similar to mammalian X-upregulation, and that Z-protein levels are fur

INSTRUMENT(S): Illumina NovaSeq 6000, BGISEQ-500

ORGANISM(S): Gallus gallus

SUBMITTER: Antonio Lentini 

PROVIDER: E-MTAB-14470 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress