SATAY sequencing data from multiple biological and technical replicates of Saccharomyces cerevisiae

ABSTRACT: Transposon insertion site sequencing (TIS) is a powerful tool that has significantly advanced our knowledge of functional genomics. While providing valuable insights, these applications of TIS focus on (conditional) gene essentiality and neglect possibly interesting but subtle differences in the importance of genes for fitness. Notably, data from TIS experiments can be used for fitness quantification and constructing genetic interaction maps, though this potential is only sporadically exploited. We aimed to develop a method to quantify the fitness of gene disruption mutants using data obtained from the TIS screen SATAY. This dataset was used to determine the reproducibility of the fitness estimates across biological and technical replicates of the same strain of S. cerevisiae. In addition,

INSTRUMENT(S): Illumina NovaSeq 6000, Illumina HiSeq X

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Enzo Kingma 

PROVIDER: E-MTAB-14476 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress