Single cell expression analysis of Defa6+ cells possessing either wild-type Tcf4 or homozygous loss of the Tcf4 protein isolated from mouse small intestinal epithelium

ABSTRACT: The T-cell factor 4 (Tcf4) protein (encoded by the Tcf7l2 gene) is a nuclear transcription regulator, which plays a key role in maintaining intestinal homeostasis, stem cell self-renewal and epithelial cell differentiation. Tcf4 has been identified as a crucial regulator of Paneth cell function in small intestinal crypts. It controls the expression of antimicrobial peptides and other effector molecules that contribute to the maintenance of the gut microbiota and protection against intestinal pathogens. The Defa6 promoter is active in adult Paneth cells and depletion of Tcf4 in these cells leads to loss of lysozyme production, altered morphology and mislocalization of these cells with the strongest phenotype in the ileum. For deeper understanding of the Tcf4 function in the Paneth cell line

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Jan Kubovčiak 

PROVIDER: E-MTAB-14489 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress