Transcription profiling of human separated leukocyte subsets in SLE and vasculitis

ABSTRACT: Objective: To optimize a strategy for identifying gene expression signatures differentiating SLE and anti-neutrophil cytoplasmic antibody-associated vasculitis that provide insight into the pathogenesis and identify biomarkers.
Methods: Forty four vasculitis patients, 13 SLE patients and 25 age and sex-matched controls were enrolled. CD4 and CD8 T cells, B cells, monocytes and neutrophils were isolated from each patient and, together with unseparated peripheral blood mononuclear cells (PBMC), were hybridised to spotted oligonucleotide microarrays.
Results: Using expression data obtained from purified cells we identified a substantial number of differentially expressed genes that were not detectable in the analysis of PBMC. Analysis of purified T cells identified an SLE-associate

ORGANISM(S): Homo sapiens

DISEASE(S): Microscopic Polyangiitis

SUBMITTER: Paul Lyons 

PROVIDER: E-MTAB-145 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress