Cancer associated fibroblasts (CAFs), CD133- (SK-BR-3 and MDA-MB-231 mixed) and CD133+ cells (SK-BR-3 and MDA-MB-231 mixed)
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ABSTRACT: CAFs were indirectly co-cultured with SK-BR-3 or MDA-MB-231 cells (CAFs, ZQY042, Zhongqiao Xinzhou Biotechnology Co., Ltd.) for one week using a 0.4um transwell chamber, after which the CAFs were harvested. FITC anti-human CD133 antibody (W6B3C1, BioLegend) was used to sort CD133- and CD133+ cells by Fluorescence activated cell sorting (FACS).
INSTRUMENT(S): Agilent TapeStation 4200, Illumina HiSeq 2000
ORGANISM(S): Homo sapiens
SUBMITTER: Kai Fang
PROVIDER: E-MTAB-14538 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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