Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of leaf tissue from four genotypes of Lathyrus sativus infected with the Erysiphe pisi

ABSTRACT: Four L. sativus accessions, exhibiting varying levels of resistance to E. pisi (resistant, partially resistant, partially susceptible, and susceptible), were analysed using dual RNA-seq. Leaflets from each plant were sampled at 0 (non-inoculated), 6, 12, 24, 48, and 72 hours after inoculation. Forty six samples were sequenced by lllumina Novaseq PE150 at STABvida sequencing provider (Lisbon, Portugal).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Lathyrus sativus

SUBMITTER: Rita Maravilha

PROVIDER: E-MTAB-14647 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

A dual transcriptome analysis reveals accession-specific resistance responses in Lathyrus sativus against Erysiphe pisi.

Maravilha Rita M RM Fernandes Telma T Barros Pedro M PM Leitão Susana T ST Rubiales Diego D Vaz Patto Maria Carlota MC Santos Carmen C

Frontiers in plant science 20250305

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering dietary benefits and desirable agronomic traits. However, its yield stability is limited by diseases such as powdery mildew caused by Erysiphe pisi. Increasing fungal resistance to pesticides and environmental concerns demand the development of resistant crop varieties. To identify key defense mechanisms and effector genes involved in the Lathyrus sativus-Erysiphe pisi interaction we ...[more]

PMID: 40110352

Similar Datasets

Transcription profiling of Arabidopsis response to Erysiphe orontii infection - AtGenExpress

Project description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana; The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Responses to E. orontii infection were assayed in wild-type Col-0 plants at 6, 12, 18, 24, 48, 72, 96, and 120 hours after inoculation of adult leaves. Inoculation was done via settling tower, using 10 day old E. orontii cultures. Leaves number 7 to 10 were selected for profiling. Experimenter name = Fred Ausubel , Julia Dewdney; Experimenter institute = AtGenExpress Experiment Overall Design: 48 samples were used in this experiment

2008-06-14 | E-GEOD-5686 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and pen3 mutant plants inoculated with powdery mildew organisms Erysiphe cichoracearum or Blumeria graminis hordei

Project description:Arabidopsis is a host to the fungal powdery mildew pathogen, Erysiphe cichoracearum, and a nonhost to Blumeria graminis f.sp. hordei, the powdery mildew pathogenic on barley. A screen for mutants that allowed increased entry by this inappropriate or nonhost pathogen on Arabidopsis led to the identification of PEN3. While pen3 mutants permitted both increased penetration and increased hyphal growth by B. g. hordei, they were unexpectedly resistant to E. cichoracearum. This resistance was correlated with the appearance of chlorotic patches and was salicylic acid-dependent. Consistent with this observation, microarray analysis revealed that the salicylic acid defense pathway was hyper-induced in pen3 relative to wild type following inoculation with either E. cichoracearum or B. g. hordei. The pen3 phenotypes result from a loss of function of AtPDR8, a ubiquitously and highly expressed ATP binding cassette transporter. PEN3 protein tagged with green fluorescent protein localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated to high levels at infection sites and surrounded fungal penetration pegs. We hypothesize that PEN3 may be involved in exporting toxic substrates to sites of infection and that accumulation of these substrates intracellularly in the pen3 mutant may secondarily activate the salicylic acid pathway. Experiment Overall Design: Three week-old wild-type Col and mutant pen3 Arabidopsis thaliana plants were inoculated with Erysiphe cichoracearum, Blumeria graminis hordei, or not inoculated. 1 day post inoculation 16 rosettes were harvested per replicate. 4 replicates were perfomerd per treatment.

2007-10-04 | E-GEOD-3220 | biostudies-arrayexpress

Cytological and molecular responses of Medicago truncatula to Erysiphe pisi

Project description:For transcript analysis of early hypersensitive and susceptible responses of Medicago truncatula to the powdery mildew pathogen, Erysiphe pisi, we compared transcripts from pathogen-inoculated and control (non-inoculated) plants 12 h after infection in resistant (A14), partially resistant (A20), and susceptible (DZA315.16) genotypes. Published in: Medicago truncatula to the powdery mildew 1 and anthracnose pathogens, Erysiphe pisi and Colletotrichum trifolii. Molecular Plant Pathology 8(3):307-319 Keywords: 1 time points and 3 genotypes

2007-01-25 | GSE6840 | GEO

RNA-seq of wild-type cells, single and double mutants of GCN5 and HDA1 grown at exponential and early post-diauxic shift phases

Project description:To determine the roles of histone acetyltransferase Gcn5 and histone deacetylase Hda1 in regulating metabolic reprogramming during glucose starvation in Saccharomyces cerevisiae cells. The transcriptome of wild-type, gcn5Δ, hda1Δ, and gcn5Δhda1Δ cells grown at both glucose-replete (exponential phase) and glucose-starved (early PDS (post-diauxic shift) phase) conditions were determined.

2023-06-01 | E-MTAB-11046 | biostudies-arrayexpress

Comparative proteomic analysis provides novel insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.)

Project description:Cucumber (Cucumis sativus L.) is an economically important vegetable crop distributed in over 80 countries. Downy mildew (DM) caused by the obligate oomycete Pseudoperonospora cubensis is especially destructive in cucumber production. So far, few studies on the changes in proteomes during the P. cubensis infection have been performed. Using a newly developed TMT-LC-MS/MS analysis, the proteomes of DM-resistant variety ‘ZJ’ and DM-susceptible variety ‘SDG’ under the P. cubensis infection were investigated. In total, 6400 proteins were identified, 5629 of which were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. KEGG enrichment analysis showed that various metabolic pathways were significantly altered under the P. cubensis infection, such as terpenoid backbone biosynthesis, and selenocompound metabolism in ZJ, and starch and sucrose metabolism in SDG. Most of the enzymes associated with terpenoid backbone synthesis were significantly accumulated in ZJ rather than in SDG, suggesting that pathogen-induced terpenoids accumulation might play an important role in the resistance against P. cubensis infection. Furthermore, a number of pathogenesis-related proteins and heat shock proteins were identified as DAPs, suggesting that DM resistance was controlled by a complex network. Our data allowed us to identify and screen more potential proteins related to the DM resistance.

2019-11-12 | PXD010844 | Pride

Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access

Project description:We describe ‘nanomaterial-biological hybrid complexes’ composed partly of an engineered nanoparticle core and formed partly by active processes within cells. Thus magnetic nanoparticles are internalized by cells, undergo active biomolecular deposition to form a new overlaid cell-derived corona layer before being re-exported. We have characterised the exported nanostructures by RNA-seq and proteomics analysis.

2026-04-16 | E-MTAB-16398 | biostudies-arrayexpress

Sclerotinia infected vs Mock infected controls in B. napus Westar and Zhong You 821

Project description:This SuperSeries is composed of the following subset Series: GSE13254: Sclerotinia infected vs Mock infected controls in B. napus (Westar) GSE13256: Zhong You 821 Sclerotinia infected vs Mock infected controls in B. napus (Zhong You 821) The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. napus oligonucleotide microarray. For each cultivar, a two-dye experiment was run comparing infected to mock-infected stem tissue. For each time point, 6 microarray slides were done (3 biological replicates, with a dye swap for each biological replicate). Refer to individual Series

2009-01-31 | E-GEOD-13262 | biostudies-arrayexpress

RNA-seq of healthy seedling leaf and root tissue of seven legume species

Project description:Seven closely related species from Hologalegina and Phaseoloid clades were selected: Lathyrus sativus, Pisum sativum (Lathyrus oleraceus), Lens culinaris, Cicer arietinum, Medicago truncatula, Glycine max, and Phaseolus vulgaris, to assess tissue-specific expression in non-inoculated conditions. Plants were cultivated in 0.5 L pots containing growth medium (50% soil, 25% vermiculite, 25% peat) in controlled environment chambers at 22°C with a 12/12-hour photoperiod. At the three fully expanded leaf developmental stage, leaf and root tissues were harvested separately, and RNA was extracted. RNA samples were submitted for Illumina NovaSeq 6000 Sequencing PE150 (40 million paired-end reads per sample) at Novogene (UK) Co. Raw reads were trimmed with cutadapt and gene counts were obtained using StringTie with the respective annotation files of each genome.

2025-11-30 | E-MTAB-15524 | biostudies-arrayexpress

Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection

Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array wheat young leveas of near isogenic lines before or 12 hours after powdery mildew infection were selected for RNA extraction and hybridization on Affymetrix microarrays.The leaf samples were harvested from three independent biological replicates, and the leaves without inoculation were regarded as control.

2011-02-15 | E-GEOD-27320 | biostudies-arrayexpress