Nanopore sequencing of B. thetaiotaomicron hankyphages

ABSTRACT: We identified hankyphage prophages within B. thetaiotaomicron isolates gathered from French hospitals. We extracted genomic DNA from an overnight culture from a single colony of each strain and sequenced them using Nanopore sequencing using the Plasmidsaurus platform. This long-read approach helped the assembly of the phages and determination of the hankyphage ends. We also improved the annotation of the reference hankyphage (hankyphage p00 from P. dorei HM719) using a structural prediction approach and annotated our B. thetaiotaomicron hankyphages using this new annotation. In this project we upload the genomic raw reads of nanopore sequencing of our hankyphage-bearing B. thetaiotaomicron collection (jmh strains) and the processed assembled hankyphages.

INSTRUMENT(S): PromethION

ORGANISM(S): Bacteroides thetaiotaomicron

SUBMITTER: Sol Vendrell 

PROVIDER: E-MTAB-14724 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress