Gene expression analysis of a Phaeodactylum tricornutum wild-type strain and a knock-out strain for the Nitrate Transporter 1/Peptide Transporter Family gene PtNPF2
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ABSTRACT: We generated a Phaeodactylum tricornutum strain in which the Nitrate Transporter 1/Peptide Transporter Family gene PtNPF2 was knocked out through the proteolistics CRISPR/Cas9 method. This strain was compared with a wild-type under normal pH conditions and after 24 hours from a shift from pH 8 to pH 7. The mutants showed a lag in growth coupled with impaired intracellular pH and photosynthetic performances.
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Phaeodactylum tricornutum
SUBMITTER: Mariella Ferrante
PROVIDER: E-MTAB-14821 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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