Project description:Part of a mouse inflammation model set in order to observe the similarities and differences between the multiple options for the generation of intestinal inflammation in an animal model. Experimental samples are generated according to the standard protocol for the model. and are processes using the same set of bioinformatic tools.
Project description:Mouse jejunum tissue sections of animals treated with Anti-CD3 antibody for the generation of intestinal inflammation. Samples of the Anti-CD3 treated mice are taken 6 and 24 hours after the administration of the antibody. Samples of animals treated with an isotype antibody are also included, with tissue taken after 24 hours of the administration. Control jejunum sections are included for comparisons.
Project description:Part of a mouse inflammation model set in order to observe the similarities and differences between the multiple options for the generation of intestinal inflammation in an animal model. Experimental samples are generated according to the standard protocol for the model. and are processes using the same set of bioinformatic tools.
Project description:Part of a mouse inflammation model set in order to observe the similarities and differences between the multiple options for the generation of intestinal inflammation in an animal model. Experimental samples are generated according to the standard protocol for the model. and are processes using the same set of bioinformatic tools.
Project description:Part of a mouse inflammation model set in order to observe the similarities and differences between the multiple options for the generation of intestinal inflammation in an animal model. Experimental samples are generated according to the standard protocol for the model. and are processes using the same set of bioinformatic tools.
Project description:Part of a mouse inflammation model set in order to observe the similarities and differences between the multiple options for the generation of intestinal inflammation in an animal model. Experimental samples are generated according to the standard protocol for the model. and are processes using the same set of bioinformatic tools.
Project description:Part of a mouse inflammation model set in order to observe the similarities and differences between the multiple options for the generation of intestinal inflammation in an animal model. Experimental samples are generated according to the standard protocol for the model and are processes using the same set of bioinformatic tools.
Project description:Colonic samples of Opa1KO mice and WT control. Mice were injected with tamoxifen for 5 days to knock out Opa1 in intestinal epithelial cells. Mice exhibited extensive epithelial damage and intestinal inflammation. On day 24, mice were sacrificed and colon was collected for RNAseq.
Project description:The Experiment was performed to understand the changes in expression profile of enteric glia cells in context of intestinal inflammation. Enteric glia are crucial regulators of gastrointestinal motility, however enteric glia alterations in IBD are poorly characterized. Acute T-cell activation rapidly impacts enteric glia activation, and induces cell death pathways, which negatively impacts small intestinal transit. This experiment has been crucial to identify IFNγ- and TNFα -driven necrosis in activated glia cell subsets. In order to sequence pure enteric glia, a mouse strain of Bl6 mice with a Plp1CreERT insert for glial specificity crossed with mice with a flox controlled tdTomato fluorescent protein on the ROSA26 Locus. The mice received a peritoneal injection of an anti-CD3 antibody or its isotype control antibody. At 6 hours, the small intestine was collected and the longitudinal muscle layer with the myenteric plexus stripped off. After enzymatic dissociation and trituration, the enteric glia were FACS sorted and finally the RNA isolated.