3' sequencing following siCFIM25 + / - DMAi, siELAVL1 or siKHDRBS1 in LU-99 cells

ABSTRACT: Knockdown of core and/or auxiliary cleavage and polyadenylation factors has been previously shown to cause global alternative polyadenylation (APA) shifts. We wanted to investigate how APA events triggered by these knockdowns compare to the APA shifts triggered by inhibition of arginine methylation, and whether arginine methylation could counteract/enhance these shifts. Therefore we performed siRNA-mediated depletion of three RNA-binding proteins - CFIM25, ELAVL1 or KHDRBS1 (Sam68), and in the case of siCFIM25 we also treated cells with arginine methylation inhibitors (100 nM LLY-283 to inhibit symmetric dimethylation and 3 μM GSK-715 to inhibit asymmetric dimethylation). siRNA-treatment was induced for 72 hours prior to harvesting, while arginine methylation inhibitors were added to cells

INSTRUMENT(S): N/A, Maxwell RSC Instrument, Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Jernej Ule 

PROVIDER: E-MTAB-14930 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress