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Single-nucleus RNA sequencing of positional airway organoids derived from human fetal lung at 4 anatomic regions (trachea, bronchus, NCA, distal)

ABSTRACT: We derived primary, epithelial-enriched organoids from 120 PCD human fetal lung, specifically from trachea, bronchus, non-cartilaginous airway, and distal airway. These positional airway organoids were grown in FANY airway media for the duration of 2 passages before collection for single-nucleus RNA sequencing (n = 1 biological replicate).

INSTRUMENT(S): N/A, Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle,, Illumina NovaSeq 6000, 10X Genomics reagent kit

ORGANISM(S): Homo sapiens

SUBMITTER: Xiangning Dong

PROVIDER: E-MTAB-14961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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