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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Shotgun sequencing of hankyphage capsids

ABSTRACT: In order to investigate the DNA packaging of the hankyphage, we performed PEG-precipitation of lysates of the B. thetaiotaomicron jmh43 strain and the jmh43::dcas9-repcHP strain (which exhibits and enhanced hankyphage induction). This was followed by a DNAse and Benzonase treatment and a subsequent SDS treatment to open the capsids and expose the DNA. Of note, jmh43::dcas9-repcHP lysate was sequenced using a PCR-free kit, whereas for jmh43 a previous step of PCR amplification was required to obtain enough DNA for Illumina sequencing.

INSTRUMENT(S): TruSeq Nano DNA Low Throughput library preparation Kit (Illumina, San Diego, USA, REF#20015964), Illumina NovaSeq 6000, PEG8000, NaCl, DNase, Benzonase, EDTA, SDS, PureGene DNA extraction Kit (Qiagen), Novogene NGS DNA Library Prep Set (Cat No.PT004), NextSeq 2000

ORGANISM(S): Bacteroides phage p00

SUBMITTER: Sol Vendrell-Fernandez

PROVIDER: E-MTAB-14979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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