Effect of hypoxia (1% oxygen for 24 hours) on the expression and transcription of human HeLa, U87, MCF7 and HCT-116 cells [RNA-seq and PRO-seq]
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ABSTRACT: Mitochondria respond to various stresses. Nevertheless, the regulation of this response while considering coordination between mitochondrial (mtDNA)- and nuclear DNA (nDNA)-encoded gene expression has been overlooked. To analyze the mito-nuclear gene expression response to hypoxia, RNA-seq was performed on HeLa, U87, MCF7 and HCT-116 cells exposed to 24 hours of 1% hypoxia and matching controls grown for 24 hours under 21% oxygen. Additionally, nascent RNA transcripts analysis (PRO-seq) in HeLa, U87 and D407 cells was performed under similar conditions.
INSTRUMENT(S): Illumina NovaSeq X, NextSeq 500
ORGANISM(S): Homo sapiens
SUBMITTER: Noam Shtolz
PROVIDER: E-MTAB-14981 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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