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RNA-seq analysis of BW25113 Escherichia coli (Wild-type, and mutants lacking the glucose metabolism regulator TmaR or the RNA Chaperone Hfq) experiencing long-term nitrogen starvation in Gutnick minimal media

ABSTRACT: RNA-seq was carried out to compare the transcriptomes of wild-type BW25113 E coli with mutants lacking either the glucose metabolism regulator TmaR or the ubiquitous RNA chaperone Hfq, in bacteria that had been exposed to long-term (24hr) nitrogen starvation in Gutnick minimal media. All strains also contain the empty plasmid pBAD18, as this was initial performed alongside a larger RNA-seq study - as such the WT and Hfq mutant are previously available in E-MTAB-14054. TmaR is also required for the formation of Hfq foci, a novel sub-cellular feature that forms during long-term nitrogen starvation. The aim of this work was to use the mutant lacking TmaR to understand the functional role of the Hfq foci during long-term nitrogen starvation.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Josh McQuail

PROVIDER: E-MTAB-14999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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