RNA-seq of primary human brain endothelial cells cultured on hydrogel substrates and exposed to static or fluid shear stress conditions

ABSTRACT: This study was designed to investigate the consequences of substrate stiffness on primary brain microvascular endothelial cells (BMECs) in the presence of fluid shear stress (FSS) of 1.7 dyne/cm2. We utilized two gelatin (GEL) substrates of different w/v% (6.5% and 15%) that were crosslinked with microbial transglutaminase resulting in a Young's modulus of 6 kPa and 30 kPa (respectively).

INSTRUMENT(S): N/A, Illumina NovaSeq 6000, Illumina Dragen RNAseq pipeline

ORGANISM(S): Homo sapiens

SUBMITTER: Alexis Yates 

PROVIDER: E-MTAB-15011 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress