SnRNA-seq of the developing human female and male gonads across prenatal development

ABSTRACT: This study used droplet-based snRNA-seq to profile the gene expression of 127,371 nuclei in the developing human female and male gonads across eleven timepoints, from Carnegie Stage (CS) 14 until 21 weeks post conception (WPC). For most timepoints the study includes two biological replicates for each sex. Additionally, the study includes three technical replicates of a Klinefelter Syndrome (XXY karyotype) testis sample at 13WPC. Human prenatal tissue samples were provided by the MRC-Wellcome Trust Human Developmental Biology Resource (HDBR) and obtained from elective terminations of pregnancy. The samples were classified according to a particular Carnegie stage or week post conception based on their external physical appearance and measurements. Single-cell barcoding and library preparatio

INSTRUMENT(S): Chromium 10x, NextSeq 550

ORGANISM(S): Homo sapiens

SUBMITTER: Nils Jonathan Trost 

PROVIDER: E-MTAB-15021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress