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Transcriptome of human HT1080 cells treated with purified typhoid toxin in the presence and absence of serum

ABSTRACT: Aim of the experiment: To examine changes in gene expression in HT1080 fibroblasts following treatment with purified recombinant wild-type typhoid toxin (WT-tox) from Salmonella Typhi relative to a mutant H160Q variant lacking DNase activity (HQ-tox), in the presence and absence of serum. The toxin causes DNA damage in both G0/G1 phase and G2/S phase of the cell cycle. Serum-starvation results in host cell cycle arrest in G0/G1 phase, meaning that DNA damage in G2/S is not permissive in the absence of serum. This experiment aimed to uncouple toxin-induced host transcriptomic responses to DNA damage in G0/G1 and G2/S phases of the host cell cycle. Experimental workflow: HT1080 fibroblasts were seeded one day before intoxication into T75 tissue culture flasks for a 30% confluency in DMEM with 1% PenStrep. Cells to be intoxicated in the presence of serum were supplemented with 10% foetal bovine serum (FBS), whereas no FBS was added for serum-starved cells. The next day, cells were incubated for 2h with WT-tox (5 ng/ml) or negative control HQ-tox (5 ng/ml) followed by three washes with PBS and a 48h chase in DMEM supplemented with 1% PenStrep and with or without 10% FBS as appropriate. After 48 h, cells were collected by trypsinisation. RNA was isolated and samples analysed using a human Clariom S assay (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems, Thermo Fisher Scientific).

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Humphreys

PROVIDER: E-MTAB-15046 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome of human HT1080 cells treated with purified Salmonella Typhi toxin

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Expression data of NIH3T3 in G0 and G1 phases

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