Transcriptome of human HT1080 cells treated with purified typhoid toxin in the presence and absence of serum

ABSTRACT: Aim of the experiment: To examine changes in gene expression in HT1080 fibroblasts following treatment with purified recombinant wild-type typhoid toxin (WT-tox) from Salmonella Typhi relative to a mutant H160Q variant lacking DNase activity (HQ-tox), in the presence and absence of serum. The toxin causes DNA damage in both G0/G1 phase and G2/S phase of the cell cycle. Serum-starvation results in host cell cycle arrest in G0/G1 phase, meaning that DNA damage in G2/S is not permissive in the absence of serum. This experiment aimed to uncouple toxin-induced host transcriptomic responses to DNA damage in G0/G1 and G2/S phases of the host cell cycle. Experimental workflow: HT1080 fibroblasts were seeded one day before intoxication into T75 tissue culture flasks for a 30% confluency in DMEM wit

ORGANISM(S): Homo sapiens

SUBMITTER: Daniel Humphreys 

PROVIDER: E-MTAB-15046 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress