Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq analysis of barley (Hordeum vulgare) single mutants in genes encoding barley CBC (HvCBP20, HvCBP80) and a double mutant in both genes encoding barley CBC in response to drought stress applied at the booting stage and subsequent rewatering

ABSTRACT: Drought poses a significant limitation to crop yield. The nuclear Cap Binding Complex (CBC), made up of CBP20 and CBP80, plays a key role in regulating pre-mRNA splicing and abscisic acid (ABA) signaling. In this study, we examined how mutations in the barley CBC genes (hvcbp20.ab, hvcbp80.b, and the double mutant hvcbp20.ab/hvcbp80.b) affect physiological and transcriptomic responses to drought stress during the booting stage. The mutants exhibited both common and unique mechanisms of drought adaptation. Transcriptome analysis showed that loss of HvCBP80 function markedly suppressed gene transcription and splicing but activated photosynthesis-associated genes, leading to improved photosynthetic performance under both normal and drought conditions. In contrast, HvCBP20 mutation enhanced the expression of ABA-responsive genes and sustained stress-related signaling. On a physiological level, hvcbp20.ab mutants maintained higher stomatal conductance despite having fewer stomata, while hvcbp80.b mutants exhibited reduced conductance under optimal conditions but formed wider stomata during drought. Although these mutations improved certain drought avoidance traits and photosynthetic capacity, they did not translate into gains in yield-related characteristics. Altogether, our results highlight the nuclear CBC as a central modulator of drought responses and recovery in barley, capable of reprogramming gene expression to support enhanced stress resilience. Here we deposit plant samples after drought stress treatment and after rewatering

INSTRUMENT(S): Macrogen, Illumina NovaSeq 6000, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice/ Thermo Fisher Scientific, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice

ORGANISM(S): Hordeum vulgare

SUBMITTER: Agata Daszkowska-Golec

PROVIDER: E-MTAB-15052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The urgent need to address water scarcity underscores the importance of enhancing plant drought resistance. This study investigates whether pretreatment with abscisic acid (ABA) activates early stress signaling, thereby improving barley drought response when subsequently exposed to drought conditions. Although the individual responses to drought and ABA are well-documented, their synergistic effects in barley warrant further investigation. This study examines the impact of ABA on barley drought resilience through an experimental design that incorporates four distinct treatments: optimal watering, ABA application at 60 days post-sowing, and two drought stress treatments - one with and the other without prior ABA application. Key physiological parameters, such as photosynthesis, stomatal conductance and chlorophyll content, were analyzed in conjunction with transcriptomics. The results suggest that ABA pretreatment initiates early stomatal closure and elevates the expression of essential genes like NCED1, BG8, and HvA22, priming barley for improved drought resistance. During the drought, ABA-pre-treated barley maintained high chlorophyll levels, indicating sustained photosynthetic activity, a trend that persisted across treatments during the post-drought recovery phase. Furthermore, ABA pre-treatment was found to preserve photosystem II efficiency during drought conditions. Transcriptomic analyses revealed distinct gene expression profiles, alternative splicing profile and isoform switching, highlighting the molecular complexities of ABA role in drought response. These alterations span stress response, metabolic pathways, and DNA modification processes, providing a comprehensive view of ABA treatment's regulatory and metabolic impacts. In conclusion, ABA pretreatment strengthens barley drought defense by fostering stomatal closure and gene activation, guiding research strategies grounded in ABA and suggesting that genotypes with elevated ABA levels could have enhanced resilience and recovery capabilities.

Dataset Information

RNA-seq analysis of barley (Hordeum vulgare) single mutants in genes encoding barley CBC (HvCBP20, HvCBP80) and a double mutant in both genes encoding barley CBC in response to drought stress applied at the booting stage and subsequent rewatering

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