RNA-seq analysis of barley (Hordeum vulgare) single mutants in genes encoding barley CBC (HvCBP20, HvCBP80) and a double mutant in both genes encoding barley CBC in response to drought stress applied at the booting stage and subsequent rewatering

ABSTRACT: Drought poses a significant limitation to crop yield. The nuclear Cap Binding Complex (CBC), made up of CBP20 and CBP80, plays a key role in regulating pre-mRNA splicing and abscisic acid (ABA) signaling. In this study, we examined how mutations in the barley CBC genes (hvcbp20.ab, hvcbp80.b, and the double mutant hvcbp20.ab/hvcbp80.b) affect physiological and transcriptomic responses to drought stress during the booting stage. The mutants exhibited both common and unique mechanisms of drought adaptation. Transcriptome analysis showed that loss of HvCBP80 function markedly suppressed gene transcription and splicing but activated photosynthesis-associated genes, leading to improved photosynthetic performance under both normal and drought conditions. In contrast, HvCBP20 mutation enhanced th

INSTRUMENT(S): Macrogen, Illumina NovaSeq 6000, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice/ Thermo Fisher Scientific, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice

ORGANISM(S): Hordeum vulgare

SUBMITTER: Agata Daszkowska-Golec 

PROVIDER: E-MTAB-15052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress