Disentangling the impact of long-term fertilization and plant diversity reduction on soil micro-eukaryotic communities using long-read sequencing.

ABSTRACT: Anthropogenic nutrient inputs alter soil biodiversity; however, it remains largely unknown whether changes in soil microeukaryotes (fungi and protists) are primarily driven by direct effects, such as modifications in soil properties, or by indirect effects, such as plant diversity loss. To disentangle these mechanisms, we investigated the long-term effects (11 years) of fertilization and manipulated plant diversity (1, 2, or 4 plant species) on soil microeukaryote communities in a temperate grassland experiment using long-amplicon rRNA sequencing. Our results indicate that fertilization generally had a stronger influence on microeukaryote communities than plant species richness. Fertilization altered the community composition of fungi and protists, increased OTU richness by 20.8% and 52.7%

INSTRUMENT(S): MinION

ORGANISM(S): mixed sample

SUBMITTER: Stefan van de Ruitenbeek 

PROVIDER: E-MTAB-15101 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress