Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of Dm-aKG treated intestinal stem cells (ISCs) and controls

ABSTRACT: The object of this study was to investigate how aKG impacts mouse intestinal stem cell (ISCs) function and fate decisions in vivo. Mice were treated for 7 days with twice daily aKG IP injections after which ISCs were FACS sorted and transcriptomically anayzled. This revealed a striking set of secretory genes upregulated in stem cells after aKG treatment.

INSTRUMENT(S): Element AVITI

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-MTAB-15105 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP172171

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Dm-aKG treated Intestinal stem cells NEBNext Enzymatic 5hmC-seq (E5hmC-seq) sequencing and data processing

Project description:To analyze aKG-induced Tet mediated changes to DNA hydroxymethylation in intestinal stem cell

2025-05-07 | E-MTAB-15115 | biostudies-arrayexpress

Development of gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation

Project description:To check gene expression signatures for LGR5hi and LGR5lo ISCs in response to gamma-irradiation, we performed whole genome microarray on LGR5hi and LGR5lo ISCs purified from irradiated mice or non-irradiated mice Radiation induced gene expression in LGR5hi and LGR5lo ISCs of LGR5-GFPki mice was measured at 3 hours after exposure to 12Gy γ-irradiation or non-irradiation.

2013-08-01 | E-GEOD-49461 | biostudies-arrayexpress

Role of polycomb repressive complex 1 in the intestinal homeostasis [ChIP-Seq]

Project description:These data include the genome wide occupancy of histone modifications and transcription factors by ChIP sequencing in mouse crypt cells and in mouse ISCs. Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type and Ring1a Ring1b dKO crypt cells and from WT ISCs using specific antibody against different target protein/modification.

2015-10-29 | E-GEOD-65318 | biostudies-arrayexpress

Aging ISCs and Polycomb KD ISCs

Project description:Aging ISCs and Polycomb KD ISCs

| PRJNA662794 | ENA

Role of polycomb repressive complex 1 in the intestinal homeostasis [ChIP-seq 2]

Project description:These data include the genome wide occupancy of histone modifications and transcription factors by ChIP sequencing in mouse villi cells and in mouse ISCs. Immuno-precipitation of formaldehyde cross-linked chromatin prepared from wild type Villi cells and wild type and Ring1a Ring1b dKO ISCs using specific antibody against different target protein/modification.

2015-10-29 | E-GEOD-71946 | biostudies-arrayexpress

Role of polycomb repressive complex 1 in the intestinal homeostasis [RNA-Seq]

Project description:These data include RNA Seq data generated from wild type and Ring1a Ring1b dKO ISCs from Lgr5Gfp-CreERT2, Lgr5Gfp-CreERT2 Ring1a-/- Ring1bf/f, Lgr5Gfp-CreERT2-Ctnnb1-ex3 and Lgr5Gfp-CreERT2 Ring1a-/- Ring1bf/f-Ctnnb1-ex3 mice. Total RNA extracted from sorted wild type and Ring1a Ring1b dKO ISCs.

2015-10-29 | E-GEOD-65321 | biostudies-arrayexpress

RNA-seq of mouse Lgr5+ intestinal stem cells and progenitors following Lkb1 deletion against controls

Project description:Perturbed intestinal epithelial homeostasis demonstrated as decreased Lgr5+ intestinal stem cells (Lgr5 ISCs) and increased secretory lineages were observed in our study where Lkb1 was specfically deleted in Lgr5 ISCs using Lgr5-EGFP-creERT2 (Tamoxifen) deletor. To gain mechanistic insight how Lkb1 maintains intestinal epithelial stem cell homeostasis, Lkb1 deficient ISCs (Lgr5-high cells) and progenitors (Lgr5-low cells) are isolated by flow cytometry and profiled by RNA sequencing to compare with controls (Lkb1 wild type ISCs and progenitors).

2020-02-29 | E-MTAB-6501 | biostudies-arrayexpress

RNAseq of Aging Drosophila ISCs

Project description:RNAseq of Aging Drosophila ISCs

| PRJNA662796 | ENA

RNAseq of Polycomb KD ISCs

Project description:RNAseq of Polycomb KD ISCs

| PRJNA662795 | ENA

RNA-seq of Dm-aKG treated intestinal stem cells (ISCs) and controls

Project description:RNA-seq of Dm-aKG treated intestinal stem cells (ISCs) and controls

| PRJEB89119 | ENA