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Gene expression profiling of RGC-32-knockdown versus control MCF-7 cells using microarray analysis

ABSTRACT: MCF-7 cells were transfected with shRNA targeting RGC-32 and a negative control. Extracting high-quality total RNA (RIN > 8.0 verified by Bioanalyzer) followed by biotin labeling.Hybridizing labeled cDNA to microarrays under standardized conditions.Scanning arrays to generate raw data files (.CEL/.IDAT).Processing data using RMA or quantile normalization followed by differential expression analysis with limma (thresholds: |log2FC|>1, adj.p<0.05).

ORGANISM(S): Homo sapiens

SUBMITTER: Peng Zhao

PROVIDER: E-MTAB-15130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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