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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Phytophthora infestans infection of three potato cultivars

ABSTRACT: Time course study of potato leaves from three cultivars (cv. Desiree, Sarpo Mira, SW-1015) 6, 24 and 72 hours after P. infestans infection

ORGANISM(S): Solanum tuberosum

SUBMITTER: Erik Alexandersson

PROVIDER: E-MTAB-1515 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Quantitative proteomics and transcriptomics of potato in response to Phytophthora infestans in compatible and incompatible interactions.

Ali Ashfaq A Alexandersson Erik E Sandin Marianne M Resjö Svante S Lenman Marit M Hedley Pete P Levander Fredrik F Andreasson Erik E

BMC genomics 20140619

<h4>Background</h4>In order to get global molecular understanding of one of the most important crop diseases worldwide, we investigated compatible and incompatible interactions between Phytophthora infestans and potato (Solanum tuberosum). We used the two most field-resistant potato clones under Swedish growing conditions, which have the greatest known local diversity of P. infestans populations, and a reference compatible cultivar.<h4>Results</h4>Quantitative label-free proteomics of 51 apoplas ...[more]

PMID: 24947944

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Three potato cultivars infected by Phytophthora infestans

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2014-06-26 | E-MTAB-1712 | biostudies-arrayexpress

Time-course Investigation of Phytophthora infestans infection of potato leaf from three cultivars by quantitative proteomics

Project description:Potato Late blight is one the most important crop diseases worldwide. Even though potato has been studied for many years, the potato disease late blight still has a huge negative effect on the potato production. A total of three commercially available field potato cultivars of different resistance to late blight infection: Kuras (moderate), Sarpo Mira (highly resistant) and Bintje (very suseptable) under controlled green house growing conditions innoculated with a diversity of P. infestans populations. We used label-free quantitative proteomics to investigate the infection with P. infestans in a time-course study over 258 hours. Several key issues limits proteome analysis of potato leaf tissue4–6. Firstly, the immense complexity of the plant proteome which is further complicated by the presence of highly abundant proteins, such as ribulose bisphosphate carboxylase/oxygenase (RuBisCO). Secondly, plant leaf and potato in particular contain abundant levels amounts of phenols and polyphenols which hinder or, unless precautions are taken, completely prevent a successful protein extraction.

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Expression analysis of Phytophthora infestans isolates 06_3928A and NL07434 during potato infection 2-4 days post-inoculation

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2018-09-03 | PXD009802 | Pride

Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection [host plant infection]

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2013-10-21 | E-GEOD-50030 | biostudies-arrayexpress

Three potato cultivars infected by Phytophthora infestans

Project description:Three potato cultivars infected by Phytophthora infestans

| PRJEB4212 | ENA

Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira)

Project description:Potato (Solanum tuberosum) late blight disease caused by Phytophthora. infestans is one of the most critical crop diseases in the world. We investigated the proteome of Solanum tubersoum (cv. Sarpo Mira) by quantitative proteomics after foliar application of encysted zoospore suspension from P. infestans isolate. Also, we examined changes in protein abundance of cv. Sarpo Mira in response to P. infestans infection at three key timepoints 0 hpi (L), 48 hpi (M) and 120 hpi (H), divided into early and late disease stages and analyzed the protein-protein interaction during the disease stages. In response to P. infestans oomycete infection, the iTRAQ comparative proteome survey revealed a total of 1229 differentially expressed proteins (DEPs) in the pairwise comparison of disease stages, which included DEPs specific to early disease stage, DEPs specific to late disease stage and commonly shared DEPs. Over 80% of the change in protein abundance were up-regulated in the early stages of infection while about 61% of the DEPs in the later disease stage were down-regulated. The analysis of expression patterns, functional category, and enrichment tests highlighted significant coordination and enrichment of cell wall-associated defense response proteins in the early stages and cellular protein modification process as well as membrane protein complex formation in the late stages. Furthermore, strong protein interactions were observed in the late disease stage that might relate to disease pathogenesis.

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